Buffer PE (Wash Buffer 2)

Final Buffer Concentration: 

• 10 mM Tris-Cl pH 7.5

• 80% Ethanol

Equipment & Consumables

• PPE: Gloves, Lab Coat and Goggles (at least!)

• Sterile Micropipettes and Tips

• Pipetteboi and tips

• Weighing Scale (accurate to to 0.01g)

  • Weigh boats (or folded paper)

• pH Meter (recently calibrated)

• Graduated Beaker

• Measuring Cylinder

• 4°C Refrigerator

• ddH20 (MilliQ or RO)

• Concentrated or pre-diluted Hydrochloric Acid (HCl)

• Pure Ethanol

Protocol:

For Mixing 400 ml of Buffer:

1. Add 0.48g Tris Base to 60mL of Autoclaved dH2O.

2. Adjust pH to 7.5 with diluted HCl.

3. Adjust volume to 80mL.

4. Add 320 mL ethanol.

5. No need to sterilise. Do not autoclave flammables!

Video: